Contact an Account ManagerPostcolumn derivatization system for the fluorimetric determination of guanidino compounds with ninhydrin by RP ion pair HPLC
Summary
Type: HPLC
Application #: 107540
Catalog#: 720990.46 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4.6x150mm
Detection: alkaline solution for postcolumn derivatization: 0.5M NaOH, 0.5ml/min; detection: fluorescence, excitation 395nm, emission 500nm
Conditions: eluent: 15mM Na octanesulphonate, 5mM ninhydrin in water - acetonitrile - methanol (92:3:5, v/v), pH adjusted with acetic acid, A) pH 4.0, B) pH 3.5
flow rate 1ml/min
Authors: Kobayashi Y
Source: J Chromatogr 400 (1987) 113 - 121
Details
Legend: Fig. 5. Chromatograms of a standard mixture of guanidino compounds. TAU =
taurocyamine (0.160nmol), GSA = guanidinosuccinic acid (0.148nmol), GAA =
guanidinoacetic acid (0.324nmol), AcARG = N-acetyl